Journal: Aging Cell

Article Title: uPAR ‐Targeting T Cell Engager Exerts Senolytic Effects in Mice and Non‐Human Primates With Serum Aminotransferase Activity as a Safety Monitor

doi: 10.1111/acel.70742

Figure Lengend Snippet: Elimination of senescent cells with mGFD‐CD3 ameliorated age‐related phenotypes in mice. (A) ELISA analysis for TNF‐α and IL‐1β in blood samples collected from mice treated with mGFD‐CD3 or mCD3 at 3 Months. ( n = 5 for young group; n = 3 for mCD3 group; n = 3 for mGFD‐CD3 group). (B) ( left ) H&E staining of IAT samples collected from mice described in (a). Quantification of adipocyte areas ( right ). Each data point represents an individual mouse. Scale bar, 50 μm. (C) ( left ) Masson staining of liver and lung tissues collected from the mice described in (a). Black arrows indicate positive staining. Scale bar, 50 μm. ( right ) Quantification of the fibrotic areas of liver or lung tissues using Image J. (D) PAS staining of kidney samples from mice described in (a). Scale bar, 50 μm (original) and 15 μm (magnified). Quantification was achieved using Image J. (E) TRAP and Masson staining of femur samples from mice described in (a). Black arrows indicate positive staining. Scale bar, 50 μm. Quantification was achieved with Image J. Data are presented as mean ± SD and analyzed by one‐way ANOVA followed by Tukey's multiple comparisons test (A–E). * p ≤ 0.05.

Article Snippet: After 24 h of coculture, the supernatant was collected and used to determine the concentrations of IFN‐γ (Invitrogen, KHC4021) and TNF‐α (Proteintech, KE00154).

Techniques: Enzyme-linked Immunosorbent Assay, Analysis, Blood/Plasma/Serum Samples, Staining

Journal: Frontiers in Immunology

Article Title: RBM15 affects mitophagy in intracerebral hemorrhage by promoting E2F1 nuclear translocation via NXF1

doi: 10.3389/fimmu.2026.1892295

Figure Lengend Snippet: NXF1 knockdown attenuates inflammatory response in vitro. (A) Expression level of NXF1 mRNA was assessed by qPCR. (B) Protein expression level of NXF1 was detected by Western blotting. (C) Cytokine levels were measured using ELISA. * P < 0.05; ** P < 0.01; *** P < 0.001.

Article Snippet: The following ELISA kits were used for detection: mouse NSE ELISA kit (Elabscience, E-EL-M0089), mouse IL-10 ELISA kit (Elabscience, E-EL-M0046c), mouse IL-6 ELISA kit (Elabscience, E-EL-M0044c), mouse TNF-α ELISA kit (Elabscience, E-EL-M3063), mouse TGF-β ELISA kit (RnDSystems, DY1679), mouse IL-1 ELISA kit (Elabscience, E-EL-M0037c).

Techniques: Knockdown, In Vitro, Expressing, Western Blot, Enzyme-linked Immunosorbent Assay

Journal: Frontiers in Immunology

Article Title: RBM15 affects mitophagy in intracerebral hemorrhage by promoting E2F1 nuclear translocation via NXF1

doi: 10.3389/fimmu.2026.1892295

Figure Lengend Snippet: NXF1 down-regulation improves the pathological changes in ICH mice. (A) Changes in lesion volume after NXF1 downregulation in ICH mice. (B) Western blotting was used to detect the expression levels of NXF1 in brain tissue. (C) HE staining was used to detect hematomas in ICH mice. Scale bar: 500 μm (40x), 200 μm (100x). (D) Changes in heart rate, blood pressure, blood glucose level, brain water content, and mNSS in ICH mice. (E) TUNEL staining was used to detect cell apoptosis. Scale bar: 100 μm (200x). (F) FJ-C staining was used to detect apoptosis in neuronal cells. Scale bar: 100 μm (200x). (G) ELISA kits were used to detect the content of IL-6, IL-1β, TNF-α, IL-10, TGF-β, and NSE. * P < 0.05; ** P < 0.01; *** P < 0.001.

Article Snippet: The following ELISA kits were used for detection: mouse NSE ELISA kit (Elabscience, E-EL-M0089), mouse IL-10 ELISA kit (Elabscience, E-EL-M0046c), mouse IL-6 ELISA kit (Elabscience, E-EL-M0044c), mouse TNF-α ELISA kit (Elabscience, E-EL-M3063), mouse TGF-β ELISA kit (RnDSystems, DY1679), mouse IL-1 ELISA kit (Elabscience, E-EL-M0037c).

Techniques: Western Blot, Expressing, Tissue, H&E Stain, TUNEL Assay, Staining, Enzyme-linked Immunosorbent Assay